ZIA BC 011186 (ZIA) | |||
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Title | Animal model of Pancreatic Cancer | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Hussain, S. Perwez | NCI Program Director | N/A |
Cancer Activity | N/A | Division | CCR |
Funded Amount | $187,124 | Project Dates | 10/01/2009 - N/A |
Fiscal Year | 2009 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Cancer (100.0%) Digestive Diseases (100.0%) |
Pancreas (100.0%) | ||
Research Type | |||
Endogenous Factors in the Origin and Cause of Cancer Application of Model Systems |
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Abstract | |||
We have established the colonies of LSL-Kras G12D (conditional K-ras mutant), LSL p53 R172 H (conditional p53 mutant), Pdx-1-Cre (pancreas-specific Cre-recombinase transgenic), NOS2- and IL-6 -deficient mice. We have also generated NOS2-deficient LSL-Kras G12D mice. To activate the conditional mutant K-ras and p53 alleles these mice are being bred with Pdx-1-Cre mice. IL-6 deficient mice are crossed with LSL-Kras G12D (conditional K-ras mutant) to generate IL-6-deficient conditional K-ras mutant mice. Following activation, the Kras and p53 double mutant mice will develop PDAC in 3-6 months in more than 90% of mice. These mice will be compared with those Kras and p53 double mutant mice that lack either NOS2 or IL-6 or are treated with anti-MIF antibody. |