ZIA CP010169 01078 (ZIA) | |||
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Title | Endogenous Hormone Measurement | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Ziegler, Regina | NCI Program Director | N/A |
Cancer Activity | N/A | Division | DCEG |
Funded Amount | $23,469 | Project Dates | null - null |
Fiscal Year | 2018 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Biochemical Epidemiology (45.0%) Cancer (100.0%) |
Breast (40.0%) Ovarian Cancer (20.0%) Prostate (10.0%) Testes (10.0%) Uterine (20.0%) |
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Research Type | |||
Endogenous Factors in the Origin and Cause of Cancer Technology and/or Marker Evaluation With Respect to Fundamental Parameters of Method |
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Abstract | |||
We have published a series of manuscripts on the reproducibility and utility of the commercial kits currently used to measure estrogens, estrogen metabolites, and androgens in blood and urine from premenopausal women, postmenopausal women, and men. While some of the assays are sufficiently reliable to discriminate among individuals, others are more problematic. In collaboration with Drs. Xia Xu and Timothy Veenstra at NCI-Frederick and Dr. Larry Keefer in the NCI Center for Cancer Research, we have developed a robust, relatively rapid liquid chromatography/mass spectroscopy procedure that can measure simultaneously 16 estrogens and estrogen metabolites in 0.5 ml of urine. A formal test of reproducibility, sensitivity, and specificity in a range of samples is being implemented. We anticipate that our approach can be extended to estrogen metabolite measurement in serum/plasma and tissue and that it can be modified to also measure androgens and phytoestrogens. |