ZIA BC 005093 (ZIA) | |||
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Title | Growth-Differentiation Factors in Organogenesis | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Perantoni, Alan | NCI Program Director | N/A |
Cancer Activity | N/A | Division | CCR |
Funded Amount | $634,620 | Project Dates | null - null |
Fiscal Year | 2018 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Cancer (100.0%) Chemotherapy (5.0%) Childhood Cancers (50.0%) |
Kidney Disease (90.0%) Urinary System (90.0%) Wilm's Tumor (90.0%) Kidney Cancer (90.0%) |
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Research Type | |||
Normal Functioning Endogenous Factors in the Origin and Cause of Cancer |
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Abstract | |||
The Renal Differentiation and Neoplasia Section studies inductive signaling in tissue development/morphogenesis and, in parallel, its dysregulation in tumorigenesis with emphasis on the ligands that mediate normal tissue interactions and the pathways and targets that are activated in response to signaling. Our focus has been on development of the urogenital tract, which features reciprocal interactions between two distinct mesodermal progenitors, highly coordinated tissue movements, mesenchymal-epithelial transition (MET), integration of structures from different lineages, reiterative cycles of development, and a tumor that caricatures nephrogenesis. More specifically we are interested in the signaling mechanisms that direct metanephric mesenchyme (MM) to convert to the epithelia of the nephron. Wilms tumor (WT) is characterized by an expanded blastemal/progenitor population with a restricted capacity for epithelial conversion (MET). It is our long-term goal to identify targets on which WT cells depend for survival or dysregulated signaling that can be reprogrammed to allow tumor cells to differentiate to a more benign phenotype. With a planned retirement around the end of this calendar year, efforts are focused on bringing current studies to a reasonable conclusion and publication. Accordingly, we have continued an assessment of cell conditions which allow for the robust expansion of cultured Wilms tumor cells for real-time testing of personalized options for therapeutic intervention. We previously defined conditions for the propagation of nephronic progenitors and applied similar conditions to studies of Wilms tumor. With minor modifications we have successfully grown Wilms tumor cells from primary tumors removed from multiple patients. These cells retain the expression of several ""stemness"" and WT-associated genes, such as, SIX2, ALDH1, and PAX2. They are stable for several passages and retain a tumorigenic phenotype based upon their ability for anchorage-independent growth. Cultured cells from these tumors have now been xenografted into NSG mice to further assess tumorigenic behavior, and we are awaiting those results. Notably, WT cells readily form organoids when placed in nonadherent culture wells, but these conditions proved inferior to monolayer cultures based upon the expression of stemness markers. Given the belief that these cells provide the driving force for WTs, the massive expansion of this population in culture witnessed in these studies may permit the development of personalized therapeutic screening for individual WT patients. This is a major advance in the field, as heretofore, failed efforts to propagate WT cells in culture have led some to speculate that they cannot be sustained except in xenografts. We continue to investigate an aberrant phenotype in the urinary tract associated with Wnt5a ablation, i.e., hydronephrosis. In these studies we show that hydronephrosis occurs as the result of a blockage in urine flow at the time renal function becomes active, causing apoptosis of the medullary region in the kidney. The blockage occurs at the interface between the ureter and bladder when cells in the common nephric duct fail to apoptose. Gene expression analysis revealed that Shh was increased in the pericloacal region in Wnt5a mutants, and we could rescue the phenotype with Shh haploinsufficiency. We also determined that constitutive activation of HH signaling yielded hydronephrosis and that Shh, which is produced by the cloacal epithelium, signals directly to the common nephric duct to sustain the tubular structure, preventing its degeneration to allow for ureter insertion into the bladder. This work demonstrates for the first time that Wnt5a suppresses Shh levels during development and possibly also during tumorigenesis, since Wnt5a expression is inversely regulated in tumorigenesis in such tissues as kidney and female reproductive tract. This work has recently been submitted for publication. We are also en |