ZIA CP010144-10376 (ZIA) | |||
---|---|---|---|
Title | Pilot Study: Collection, Analysis of Human Ovarian Surface Epithelial Cells | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Wentzensen, Nicolas | NCI Program Director | N/A |
Cancer Activity | N/A | Division | DCEG |
Funded Amount | $39,509 | Project Dates | 02/01/2006 - N/A |
Fiscal Year | 2012 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Biochemical Epidemiology (45.0%) Cancer (100.0%) |
Ovarian Cancer (50.0%) Uterine (50.0%) |
||
Research Type | |||
Cancer Related Biology Endogenous Factors in the Origin and Cause of Cancer |
|||
Abstract | |||
We propose to systematically assess the prevalence of molecular markers in ovarian tissues that are collected incidentally at the time of hysterectomy for non-malignant conditions. In the current study, the collection procedure will be modified by utilizing a novel laparoscopically deployed cytobrush to collect the cells, and performing the OSE harvest prior to removing the ovaries from the pelvis, in order to minimize the loss of epithelium due to handling of the specimen, and prior to vascular ligation, in order to minimize ischemia-related changes in the samples collected. This study opened to patient enrollment in October 2008 and has, to date, accrued 50% of the planned samples. In addition, the assessment of epidemiologic data in combination with the proposed molecular assays, may provide an important model for assessing biomarkers in the ovary. These data are important for assessing the sensitivity, specificity, and predictive value of candidate biomarkers for ovarian cancer. Our scientific hypothesis in this methods-oriented proposal is that etiologically-relevant silent molecular lesions will be more prevalent among women with specific epidemiologic risk factors for ovarian cancer than in women who lack those risk factors. Prelimary data suggest a marked increase in the number of epithelial cells obtained per ovary, and a signficant reduction in red cell contamination of the samples, compared with the collection protocol used in prior studies. Modification of our cell collection procedure using the Tao brush and only ligating the ovarian vasculature after the cells have been collected has succeed in producing large numbers of relatively pure epithelial cells. Pilot evaluation of proteomic expression arrays and oligonucleotide expression arrays have been performed and demonstrate that satisfactory reagents have been obtained. Now that 75 samples have been collected, the laboratory work has begun in earnest. |