ZIA CP010120 02093 (ZIA) | |||
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Title | Disinfection Biomarker | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Beane Freeman, Laura | NCI Program Director | N/A |
Cancer Activity | N/A | Division | DCEG |
Funded Amount | $90,516 | Project Dates | 11/21/2005 - N/A |
Fiscal Year | 2010 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Biochemical Epidemiology (45.0%) Cancer (100.0%) |
Bladder (100.0%) | ||
Research Type | |||
Exogenous Factors in the Origin and Cause of Cancer Endogenous Factors in the Origin and Cause of Cancer |
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Abstract | |||
We are assessing the influence of enzyme variants (genetic polymorphisms) and other exogenous and endogenous factors on the increase of THM in the blood of people exposed to DBP while showering. From a pool of pre-screened individuals with a normal standard blood panel and for genetic polymorphisms of interest, we recruited approximately 100 people who have enzyme variants of differing activity. We asked the 100 volunteers to provide seven 10- mL blood samples and two urine samples, and take a 10-minute shower at the study site (the CCP in Pittsburgh, P A). Blood samples have been analyzed for THM concentrations, and red blood cell enzyme activities. To study the activity of the enzyme CYP2El, we administered a single dose of chlorzoxazone, a muscle relaxant metabolized by this enzyme. We are measuring enzyme activity by analyzing blood samples collected 2 hours post-administration. We conducted a brief interview with each volunteer to obtain demographic and other information that might impact the dose of THM. We collected ambient air samples before, during and after showering for each participant and analyzed them for THMs. A water sample was collected during showering and analyzed for levels of THM. Levels of THM in blood before and after showering, and the rate of decrease in blood concentration, are being analyzed with respect to the presence of genetic polymorphisms for selected enzymes, or their phenotypic activity .Blood THM levels will also be compared with various demographic and physiologic measurements. To test intra-individual variation in several measures, ten randomly selected participants (stratified by sex, i.e. 5 males and 5 females) were asked to conduct the study twice, with the two study appointments separated by at least a week. |